According to the previous described method [18], the latency of the rotarod test was used to evaluate motor coordination and balance. The rotarod test was performed 4 times at 6 days intervals after EAE induction. Each rat was placed in a separate compartment on the rotating rod (diameter: 7 cm, fixed velocity: 30 rpm) of the rotarod apparatus (Biological Research Apparatus, Ugo Basile, Varese, Italy). The latency time until fall was automatically recorded by magnetic trip plate. To eliminate stress and fatigue, we limit the latency time for rats to 300 seconds.
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