Analysis of Therapeutic Effect on Xenograft Model in vivo

The MDA-MB-231 cells (5×10⁶/mL) were collected in a serum-free L-15 medium (200 μL) and subcutaneously injected into the right forelimb axilla of female BALB/c nude mice. After one week, nude mice bearing a MDA-MB-231 tumor with a volume of approximately 120 mm³ were randomly divided into six groups and injected with saline, 10 mg/kg PTX, 30 mg/kg PTL+ 2 mg/kg ICG (-), 30 mg/kg PTL+ 2 mg/kg ICG (+), PI-TSL (-) containing 30 mg/kg PTL and 2 mg/kg ICG, or PI-TSL (+) containing 30 mg/kg PTL and 2 mg/kg ICG via the tail vein every two days for 19 consecutive days. Both, the tumor volume and the body weight of the mice were examined every two days. The mice were sacrificed at the end of the experiment and the major organs and tumors were harvested. The samples were collected and stained with hematoxylin-eosin staining (H&E) for analysis. Tumor tissues were further subjected to immunohistochemistry for Ki67 evaluation.