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Complement-Dependent Cytotoxicity (CDC) Assay

RS Ramsey Saleem
GC Greg Cantin
MW Mats Wikström
GB Glen Bolton
SK Scott Kuhns
HM Helen J. McBride
JL Jennifer Liu
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The CDC activity of ABP 710 was evaluated in a functional cell-based assay. Target CHO MT-3 cells expressing mbTNFα were loaded with the fluorescent dye, calcein-AM. The calcein-AM loaded target cells were incubated with different dose concentrations of reference standard, control and test sample(s) followed by addition of rabbit complement. After allowing for complement-mediated cell lysis to take place, the amount of cellular lysis induced by the antibody was measured using a fluorimeter. Dose-response data were fit to a 4-parameter curve, and the EC50 values derived from the curves were used to calculate the test sample relative activity.

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