Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Cellular Assays—ERK Phosphorylation, CREB Phosphorylation, and Akt Phosphorylation
Ask a
question
Favorite
AlphaLISA SureFire Ultra kits were used to measure ERK phosphorylation on residues T202/Y204, CREB phosphorylation on S133, and Akt phosphorylation on S473. Assays were performed in accordance with previous literature.41 Stimulation durations for concentration–response experiments were 10 min in all instances, otherwise the protocol remained unchanged. For Akt phosphorylation, 50% FBS, and 200 nU insulin were used as positive controls.
Copyright and License information: ©2020 American
Chemical Society
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
