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Preparation of total T-cells and T-cell-depleted BM
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Total T-cells were isolated from naïve C57BL/6 spleens by negative selection using mouse Pan T-Cell Isolation Kit II (Miltenyi Biotec, Auburn, CA, USA) with a purity of >97%. T-cells were depleted from BM cells using the CD3ε MicroBead Kit (Miltenyi Biotec), with less than 0.3% CD3ε+ cells remaining.
Copyright and License information: ©2018-10-13
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