3.7. Wound Healing Assay

To analyze cell migration, the wound healing scratch assay was performed on untreated control cells and cells treated with AuQDs and soft jet plasma. The cells were cultured to 100% confluence in 96-well plates and serum-starved after 8 h of seeding until the end of the experiment. At 95% confluence, a scratch wound was made on the cell culture using a Sartorius wound maker, followed by treatment. Air soft jet plasma (200 s)-treated medium was mixed with 25 mM AuQDs and used for treatment. After the treatment, the plates were incubated in a Sartorius Incucyte Korea, and cellular migration was scanned and captured every 2 h using Sartorius Incucyte software (https://www.essenbioscience.com/en/products/software/incucyte-base-software/) After 2 days, image scanning was stopped for data analysis using Incucyte software.