Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Preparation of Lung Homogenates and Cytokines Assays

KW Ke Wei
JL Jie Luo
JC Jun Cao
LP Lihua Peng
LR Li Ren
FZ Fan Zhang
ask Ask a question
Favorite

The intermediate lobes of the right lungs were harvested and homogenized in 1 mL of PBS with an Ultra-Turrax tissue homogenizer. The homogenates were then centrifuged at 2000 rpm for 10 min at 4°C. The supernatants were passed through a 0.45 µm filter, collected, and then stored at −80°C for cytokine production analysis. The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 in the lung homogenate supernatants were determined using the enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Copyright and License information:  ©2020 Wei et al.
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A