2.2. Normal Brain Lysates, Human Glioma Cell Preparation, and Western Blot Analysis

The human GBM cell lines GBM8401, LN229, U87MG, U118, and T98G were obtained from the laboratory room of Dr. Ying-Chuan Chen, National Defense Medical Center. GBM8401 was maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 5% fetal bovine serum (FBS). LN229, U87MG, and U118MG were maintained in DMEM with 10% FBS. T98G was maintained in F12/DMEM with 10% FBS. Human umbilical vein endothelial cells (HUVECs) were maintained in the extracellular matrix (ECM) containing basic fibroblast growth factor. The cell lysates were prepared from 2 × 10⁷ cells GBM cell lines. Normal human brain lysates were purchased from GeneTex, Irvine, California, USA. Western blot (WB) assay protocol was followed as previously described [18]. All primary antibodies and their detailed information are listed in Supplementary Table S1.