Spectrum of Inhibition

The spectrum of inhibition was completed on overnight cultures of 29 antimicrobial-producing isolates with a distinct immunity profile using 29 distinct indicator strains by a spot bioassay as described above with small modifications. More precisely, 5 μL of an overnight culture of the 29 antimicrobial-producing isolates were spotted on a clean TSA plate and incubated aerobically overnight at 37°C, before UV irradiation for 45 min. The rest of the procedure was performed as described above. The indicator strains employed to detect antimicrobial activity and their optimal growth conditions are listed in Table 1. Anaerocult A gas packs (Merck, Darmstadt, Germany) were used to generate anaerobic conditions where necessary for a selection of the indicator strains used in the study. Zone size was measured as follows: diameter of zone – diameter of spot in millimeters. The experiment was performed in three independent replicates.

Growth conditions for the indicator strains used in this study.