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Tissue processing and RNA extraction

SL S. Lee
SB S. J. Bush
ST S. Thorne
NM N. Mawson
CF C. Farquharson
GB G. T. Bergkvist
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Samples including teeth, mandibles and maxilla were snap frozen in liquid nitrogen and stored at − 80 °C until RNA extraction. Information of samples are described in Table ​Table1.1. Teeth were extracted from the alveolar sockets using dental equipment or using bone cutters while maintaining cold temperatures by working over dry ice. Tissue processing and RNA extraction protocols were optimised based on a guanidinium thiocyanate-phenol–chloroform extraction method and manufacturer’s instructions for the Qiagen RNeasy Mini Kit. This protocol has been previously documented28.

Phenotyping of TR and selection of samples for RNA sequencing.

TR-tooth

from TR + cat

RINe: the RNA integrity number equivalent, N/A: not applicable, F: female, N: RNA sample number, C: cat number, FN: female neutered, RNA yield shows total yield. PM; premolar, M; molar, C; canine.

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