Preparation of test solutions

The test solutions were prepared in HBSS and given to the mice in volumes of 250 μl, resulting in the following doses per animal per gavage: A; 5.7 mg Lupex, B; 5.7 mg Lupex + 10 μg CT, C; 5.7 mg Lupex + 10 μg CT + 10 μg trypCry1Ab, D; 5.7 mg Lupex + 10 μg trypCry1Ab, E; 10 μg trypCry1Ab, F; 10 μg trypCry1Ab + 10 μg CT. The control solution G consisted solely of 250 μl HBSS. A high dose of 10 μg trypCry1Ab was selected due to the proof-of-principle character of the study and in order to be able to compare a potential adjuvant effect with the effect induced by 10 μg of the positive control, CT. Restrictions in gavage volume (max 250 ml) and the trypCry1Ab solubility prevented us from giving a higher dose per animal per exposure.