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4.7. 3D Spheroid Assays

IM Idoya Martínez-Vicente
MA Marta Abrisqueta
CH Cecilia Herraiz
JS Julia Sirés-Campos
MC María Castejón-Griñán
DB Dorothy C. Bennett
CO Conchi Olivares
JG Jose Carlos García-Borrón
CJ Celia Jiménez-Cervantes
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Spheroid cell culture was performed using the hanging drop method, adapted from Del Duca et al. [67]. Briefly, spheroids of melan-a6 or melan-md1 cells were collected and resuspended in a collagen I solution (1.7 mg/mL in DMEM), added on top of a thin layer of previously polymerized collagen I. For growth quantification, increase of the area of spheroids between day 0 and 4 was calculated using ImageJ.

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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