2.6. Intraperitoneal glucose tolerance test (IP GTT)

After 8 and 16 weeks of diet consumption, a glucose tolerance testing was performed. Male and female mice were fasted for 6 h, blood was collected by tail vein puncture and the droplet analyzed by standard glucometer (TRUEresult, Nipro Diagnostics). Following baseline readings, all mice were given injections of glucose (1.5 g/kg, IP). Blood was tested at 15, 30, 60 and 120 min post-injection. Following each test, pressure was applied to the puncture to stop bleeding and mice were returned to their home cage.