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2.4. microRNA Inhibitor Transfection
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Anti-miR miRNA inhibitor for has-miR-106b (ThermoFisher Scientific, Assay ID AM10067, #AM17000, Waltham, MA, USA) and anti-miR miRNA inhibitor negative Control #1 (ThermoFisher Scientific, #AM17010, Waltham, MA, USA) were used to transfect the 293T cells transduced with LV-dt/EGFP-miRT. The transfection procedure followed the RNAi transfection protocol provided with Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific, #13778100, Waltham, MA, USA).
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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