Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Sea urchin collection

JS Juliana Mozer Sciani
AE Andrews Krupinski Emerenciano
JS José Roberto Machado Cunha da Silva
DP Daniel Carvalho Pimenta
request Request a Protocol
ask Ask a question
Favorite

Sea urchins (Echinometra lucunter, Lytechinus variegatus and Arbacia lixula) were collected in São Sebastião, SP, Brazil, under license number 13852–1 from the Brazilian Institute of Environment and Renewable Natural Resources (IBAMA) and in partnership with the Center for Marine Biology (CEBIMar/USP).

Animals were collected without distinction of gender, age or size. The coelomic fluid (circa 30 mL) was extracted from the sea urchin by puncturing the peristomial membrane. Spines were removed and immersed in a buffered solution (ammonium acetate 100 mM, pH 7.3) for molecule extraction for 24 h, at 4 °C.

Copyright and License information: Sciani et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A