CCl4 induced acute liver injury

Bean oil (0.2 ml/10 g body weight) was administered to the mice in the control group and the mice in other experimental groups were given 2% CCl₄ dissolved in plant oil (0.2ml/10g) by gavage every morning. The administered dose of CCl₄ is well-known in the literature to induce characteristic liver injury in mice that functionally resembles the acute liver injury observed in humans [26] (http://d.wanfangdata.com.cn/Periodical_xjykdxxb200711006.aspx). Three hours after CCl4 administration, the low dose, moderate dose and high GSP dose groups were given GSPs by gavage suspended in distilled water at 50, 250, 500 mg/kg body weight, respectively. The dose of GSPs used in this study was selected on the basis of previous studies [27]. The DDB group was given DDB at 150 mg/kg body weight by gavage [28], while the control group and CCl₄-model group were given same volume of saline. As per standard protocols, the experimental procedures were continued for 10 days until significantly elevated serum alanine aminotransferase levels were detected [26].