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Histochemistry and immunohistochemistry staining
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Penile tissue sections were prepared as aforementioned and adjacent tissue sections were used for: (a) Masson trichrome staining for collagen (blue) and smooth muscle cell (red) (Muto Pure Chemicals, Tokyo, Japan); (b) immunodetection with: (i) polyclonal antibody against iNOS (Calbiochem, La Jolla, CA, USA); (ii) rabbit polyclonal anti-eNOS IgG antibody against eNOS (Santa Cruz Biotechnology, Santa Cruz, California, USA). All the staining procedure were carried out according to the manufacturer’s instructions.
Copyright and License information: ©2017 Shang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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