Cell culture and transfection

Human lens epithelial cells SRA01/04 were purchased from the Cell Resource Center of the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (http://cellresource.cn/contact.aspx) (China) and cultured in DMEM medium (Gibco, USA) supplemented with 10% FBS (Gibco) and 0.05% penicillin/streptomycin. miR-378a-5p mimic (miR-378a-5p), miR-630 mimic (miR-630), miR-378a-5p inhibitor (anti-miR-378a-5p), miR-630 inhibitor (anti-miR-630), negative control (miR-NC), and negative control inhibitor (anti-miR-NC) were purchased from RIBOBIO (China). Small interfering RNA (siRNA) targeting E2F3 (si-E2F3), negative control (si-NC), pcDNA E2F3 overexpression vector (pcDNA-E2F3), and pcDNA negative control (pcDNA-NC) were synthesized by Genepharma (China). Cell transfection was performed using Lipofectamine 2000 (Invitrogen, USA).