Luciferase reporter assays

YC Yuji Chikashige
HK Hiroaki Kato
MT Mackenzie Thornton
WP Whitney Pepper
MH Madelyn Hilgers
AC Ariana Cecil
IA Izumi Asano
HY Haana Yamada
CM Chie Mori
CB Cheyenne Brunkow
CM Carter Moravek
TU Takeshi Urano
CS Chingakham Ranjit Singh
KA Katsura Asano
request Request a Protocol
ask Ask a question
Favorite

S. pombe transformants carrying the dual luciferase plasmids were grown in EMMC-His-Leu and treated with 10 mM 3AT. Fixed A600 units of culture were collected and subjected for Dual GloR Luciferase Assay System, as described in Supplementary Materials and Methods.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A