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In brief, CM was added to an equal volume of DMEM in the first column of wells in a 96-well microtiter plate and titered in 1:2 dilutions in DMEM. One-N-phenylaphthylamine (NPN) (Sigma-Aldrich) was added to each well for a final concentration of 10 μm, and 50 μl of bacteria from cultures in the exponential growth phase were added. Plates were analyzed immediately in an Infinite 200 pro plate reader (Tecan) using an excitation of 355 nm and emission of 444 nm.
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