3. ZMTH3 cell culture

For in vitro testing of imaging and ablation before moving to resected organs and in vivo experiments, cultures of the canine pleomorphic mammary adenoma cell line ZMTH3 were used. Cells were cultured in petri dishes in supplemented RPMI medium with the selection antibiotic G418 and split when necessary (all reagents PAN-Biotech GmbH, Germany). Cells expressed mitoDsRed, a red fluorescent protein (RFP), which is tagged to mitochondria, and were stained with Calcein AM (ThermoFisher, US).