The study was prospective, parallel-grouped with animals randomized through the sealed envelope technique into four treatment groups (I) bacteria killed by cefuroxime, (II) live bacteria, (III) bacteria killed by heat, and (IV) bacteria killed by the combination of cefuroxime and tobramycin. Each group was comprised of four animals. By killing the bacteria ex vivo and with the removal of the supernatant, the effect of antibiotic-induced structural changes could be studied without the influence of the endotoxin that is liberated during the killing process [7, 9, 24].
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