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Virus-mediated cytotoxicity assays

LY Lei Yin
CZ Chunhong Zhao
JH Jixia Han
ZL Zengjun Li
YZ Yanan Zhen
RX Ruixue Xiao
ZX Zhongfa Xu
YS Yanlai Sun
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The cytopathic effect was evaluated by viral cytotoxicity, cell cycle progression, and apoptosis analysis. The effect of oHSV2 on the proliferation of cancer cell lines was evaluated using the MTT assay (AMRESCO LLC, Solon, OH, USA) according to the manufacturer’s instructions. Cell viability was determined by measuring absorbance at 570 nm with a reference of 630 nm using a microplate reader (BIO-RAD, Hercules, CA, USA). The controls on each day were set at 100% viability. The percentage cell survival rate treated with oHSV2 was calculated using the following formula: (100%× [absorbance value of experimental cells]/[absorbance value of control cells]). All measurements were performed in triplicate.

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