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Muscle ATP extraction and ATP quantification
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ATP was extracted from frozen sections of TA muscles. Tissue was homogenized with 1 ml of ice-cold phenol-chloroform-isoamylalchol (25:24:1). 200 µl of PBS were added to the homogenate, and the latter was centrifuged at 14000 rpm for 10 min at 4 °C for phase separation. 20 µl of the upper aqueous phase was used to measure ATP. ATP measurements were performed with ATPlite Luminescence Assay System (Perkin Elmer) according to manufacturer’s instructions. Final results were normalized for muscle weight.
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