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ZIKV infection in human brain organoids
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The superficial cell number in organoids was calculated by dividing the superficial area (calculated using: 4πr2) by the mean cell area in the organoid surface (15 μm2). Brain organoids were infected using ZIKV MOI 0.5 (2–6.5 x 105 PFU per organoid)—for 2 h, then cultured in medium with (or without) STX 12 μg/L (NRC Halifax, CAN) for 13 days. Mock-exposed organoids (treated and non-treated with STX) were used as control. The assay was performed in triplicates in three independent experiments.
Copyright and License information: ©2020 Pedrosa et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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