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To measure PGE2 levels according to the treatment used; media was collected from the corresponding samples at 96 h of treatment and was processed using the Prostaglandin E2 Parameter Assay Kit (KGE004B, R&D Systems, St. Cloud, MN, USA). Optical density was determined using a microplate reader set to 450 nm. Evaluation was carried out as per manufacturer's instructions. Standards and controls were included as described in the kit.
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Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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