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Spectra analysis and reference database creation

SN Sirama Niare
JB Jean-Michel Berenger
CD Constentin Dieme
OD Ogobara Doumbo
DR Didier Raoult
PP Philippe Parola
LA Lionel Almeras
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The reproducibility of MALDI-TOF MS spectra from mosquito specimens engorged on the same vertebrate and collected at different points in time was evaluated by comparing the average spectra obtained from the four spectra of each sample tested using the Flex analysis and ClinProTools 2.2 software (Bruker Daltonics). Specificity of MALDI-TOF MS spectra according to blood host origin was also analysed using the Flex analysis and ClinProTools 2.2 software (Bruker Daltonics). To create an MS database, reference spectra (MSP, Main Spectrum Profile) were created by combining the results of the spectra from at least three specimens per species that were either unfed or fed on the same vertebrate blood, and harvested at the same point in time by the automated function of the MALDI-Biotyper software v3.0. (Bruker Daltonics). MSP were created on the basis of an unbiased algorithm using information on the peak position, intensity and frequency.

Copyright and License information: Niare et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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