Cell proliferation assay

LNCaP cells (750 cells/well) or 22Rv1 cells (1,000 cells/well) were seeded in 96-well plates and allowed to attach. The attached cells were treated with ethanol (control) or the indicated doses of LLM, Etomoxir or Cerulenin for 24, 48 or 72 hours. Subsequently, 20 µL of manufacturer supplied colour development reagent was added to each well and the plates were incubated at 37°C for 2 hours. Absorbance was measured at 492 nm.