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Acid-insoluble lignin was determined using Klason lignin analysis of 150–200 mg extractive-free rind tissue, as previously described [15]. Acid-soluble lignin was determined by measuring the absorbance at 205 nm of the filtrate after isolation of acid-insoluble lignin and calculated using an extinction coefficient of 110 L/g cm.
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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