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The behavioral response to being touched by an eyelash was adapted from an assay previously described [53, 54]. The animals were touched anteriorly specifically behind the terminal bulb of the pharynx with the eyelash, 10 times per animal, with a 10 s gap between each touch. Typically, if the animal demonstrates an omega turn or if it reversed its direction after an anterior touch, the animal was scored as giving positive response. Touch response percentage was generated by the amount of times an animal responded to the touch stimulus over the total number of times they were touched.

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