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The 25CN-NBOH hydrochloride (a kind gift from Jesper L. Kristensen, University of Copenhagen) was dissolved in isotonic saline and applied subcutaneously (<10 mL/kg). The dose used (1.5 mg/kg) was found optimal for a 5-HT2AR-specific effect, as determined by a dose-response curve for 25CN-NBOH-induced head twitches (Buchborn et al., 2018). Experiments were performed under general anaesthesia using isoflurane (IsoFlo; Zoetis, UK) delivered in 100% oxygen, as detailed below.
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This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
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