Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Comparison of mitochondrial mass using nonyl acridine orange (NAO)
Request a Protocol
Ask a
question
Favorite
Cells were seeded in 6 cm dishes and treated with 100 nM NAO the following day. After 15 minutes of incubation, cells were harvested by trypsin and pelletized. After one washing step in in PBS, mitochondrial staining of 100,000 cells per sample was analyzed via FITC channel with the BD FACS Canto II flow cytometer and the BD FACSDiva software (BD Biosciences, Franklin Lakes, USA). Data were evaluated using FlowJo software (FlowJo LLC, Ashland, USA) and related to MCF-7/empty control cells.
Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
