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Inositol phosphate analysis by SAX-HPLC
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Analysis of InsP pathways after [3H]-inositol radiolabeling was carried out as previously described25. Briefly, cells were seeded into 6-well plates and grown in the presence of [3H]-inositol for 5 days to ~80% confluence. Treatment with NaF (10 mM) was for 1 h. Cells were then washed with ice-cold PBS and extracted with perchloric acid, and after neutralization processed for SAX-HPLC analysis.
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