Hyperbaric oxygen therapy

Hyperbaric oxygen therapy was performed 2 h after TBI, as previously reported [4]. The rats were placed into the animal chamber, which was purged with pure oxygen for 10 min to ensure that the oxygen fraction in the chamber was >95%. The pressure was then steadily increased to 0.12 MPa and maintained for 60 min. Next, the pressure was steadily decreased to normal pressure over 20 min. Hyperbaric oxygen therapy was performed twice with a 10 h interval. The rats’ behavior in the high-pressure chamber was closely monitored. The sham control group and untreated TBI group were also placed in the same chambers and were subjected to the same experimental procedures, only without the hyperbaric oxygen treatment.