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Base catalyzed hydrolysis for epoxide ring-opening

JY Jong-Jae Yi
SH Sun-Yeon Heo
JJ Jung-Hyun Ju
BO Baek-Rock Oh
WS Woo Sung Son
JS Jeong-Woo Seo
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The epoxide ring-opening reaction was carried out using sodium hydroxide (NaOH) as a strong nucleophile. The aqueous solution for the reaction was prepared to contain 0.2 M NaOH in distilled water (DW), to which was added epoxidized material at a final concentration of 100 μg mL−1. The reaction mixture was incubated overnight at 30 °C with stirring. As a reference, the hydrolysis reaction for epoxide ring opening was performed using a commercially available EH from Rhodococcus rhodochrous (Sigma-Aldrich) in accordance with the manufacturer’s protocol. The hydrolyzed products were extracted using HP20, as described in the enzyme reaction section.

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