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In situ hybridization and immunohistochemistry

AR Arsheen M. Rajan
RM Roger C. Ma
KK Katrinka M. Kocha
DZ Dan J. Zhang
PH Peng Huang
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Whole-mount in situ hybridization and antibody staining were performed according to standard protocols. col1a2 and col5a1 antisense probes were used in this study. Double fluorescent in situ hybridization was performed using digoxigenin (DIG) and dinitrophenyl (DNP) labeled probes. For antibody labeling, the rabbit polyclonal antibody to GFP (1:500, MBL) was used. For fluorescent detection of antibody labeling, appropriate Alexa Fluor-conjugated secondary antibodies were used (1:500, Thermo Fisher).

Copyright and License information:  ©2020 Rajan et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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