In vivo biodistribution

YK Yi Wen Kong
ED Erik C. Dreaden
SM Sandra Morandell
WZ Wen Zhou
SD Sanjeev S. Dhara
GS Ganapathy Sriram
FL Fred C. Lam
JP Jesse C. Patterson
MQ Mohiuddin Quadir
AD Anh Dinh
KS Kevin E. Shopsowitz
SV Shohreh Varmeh
ÖY Ömer H. Yilmaz
SL Stephen J. Lippard
HR H. Christian Reinhardt
MH Michael T. Hemann
PH Paula T. Hammond
MY Michael B. Yaffe
request Request a Protocol
ask Ask a question
Favorite

Tumor accumulation was approximated using a Xenogen IVIS Imaging System (Caliper). Tumor-bearing mice (n = 3) were intraperitoneally injected with nanoparticles (1 mg kg−1, AllStars Negative Control siRNA, Alexa Fluor 647; Qiagen) dispersed in phosphate-buffered saline (PBS). After 24 h, mice were anesthetized with isoflurane and imaged (640/700 nm ex/em) using aperture-limited transillumination fluorescence tomography. The signal in tomographic images was spatially limited to that from the upper abdomen. Biodistribution measurements were taken from whole animal epifluorescence images. Recovered fluorescence from the lungs and whole animal were quantified using the region-of-interest analysis package in Living Image (Perkin Elmer).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A