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RNA-Binding Protein Immunoprecipitation (RIP)
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RIP assay was used to determine the binding between ROR1-AS1 and miR-4686 using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore) as in previous study.23 Briefly, U251 cells were transfected with biotinylated miR-4686 or miR-NC, and the expression of ROR1-AS1 was detected using qRT-PCR.
Copyright and License information: ©2020 Chai et al.
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