2.4. Motor Performance and Coordination in Rotarod Test

Motor performance and balance were evaluated by means of a rotarod (Panlab^®). Animals performed training sessions during five days prior to the test (one session/day) on the rotarod at a constant speed (4 rpm) until they attained a stable performance. On the test day, the rats were placed on the rotarod in acceleration mode (from 4 to 40 rpm over a period of 60 s) in order to evaluate their latency to fall down. Each rat repeated the test five times, leaving some minutes for recovery between tests. The mean measured was used as the motor coordination value. The rotarod design was performed at the beginning of the treatments (t0) and after the 30 days of the treatments (t30).