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Operetta imaging and IncuCyte analysis

JZ Jing Zhang
YY Yu Yang
SZ Shen’ao Zhou
XH Xueyan He
XC Xuan Cao
CW Chenlu Wu
HH Hong Hu
JQ Jie Qin
GW Gang Wei
HW Huayi Wang
SL Suling Liu
LS Liming Sun
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For Operetta imaging, L929 Tnfr1 KO cells stably expressing EGFP and L929 Tnf KO cells were seeded in 96-well plate at the ratio of 5:1, then treated with 500 nM NCZ for 24 h, and stained with PI for 10 min. Images were taken by operetta (PerkinElmer) using the Harmony software. EGFP was imaged at 460–490/500–550 nm and PI was imaged at 520–550/560–630 nm. For IncuCyte dead cell analysis, L929 Tnf KO and L929 Tnfr1 KO cells were seeded in 96-well plated as indicated. Cells were treated with DMSO or 500 nM NCZ for 24 h and stained with 100 nM SYTOX Green. Cells were imaged (450–490 nm/500–530 nm) and quantified by IncuCyte FLR.

Copyright and License information: The Author(s) ©2019
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