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4.4. Histological Assessment
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Pieces of the liver were fixed in 4% paraformaldehyde and processed to paraffin wax then stained with hematoxylin and eosin (H&E). In addition, lipid droplets in the liver were observed by ORO staining in the frozen liver sections and quantified by ImageJ software. Histopathological changes of the livers were observed and photographed under a light microscope (Olympus, Tokyo, Japan).
Copyright and License information: ©2020 Xiaoqin Ding et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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