After respiratory mechanics, anesthesia was closed, and ventilation was performed using only ambient air. After 5 min, a mylar balloon was connected to the FlexiVent expiratory way, and NOex was collected for 3 min [8,13]. The NOex concentrations were measured by chemiluminescence using a fast-responding analyzer (NOA 280, Sievers Instruments, Inc., Boulder, CO, USA).
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