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Tissues were first fixed in paraformaldehyde and then embedded using paraffin. After that, sections (5 μm) were cut. For IHC assay, the antigen was repaired after tissue sections were rehydrated. After that, antibodies for Ki-67, Notch1 and Notch2 were used to incubate tissue sections overnight at 4 °C. Then, corresponding secondary antibodies and 3, 3ʹ-diaminobenzidine (DAB) solution (Sigma-Aldrich, St. Louis, MO, USA) were used to visualize the sections.
Copyright and License information: ©2020 Wang et al.
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