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Adipose tissue biopsies were performed under local anesthesia after all PET scannings had been performed. Abdominal SAT was biopsied 6–8 cm laterally from the umbilicus under local anesthesia. For the gene expression analyses, the tissue sample was snap frozen in liquid nitrogen and stored at −70°C. For adipocyte cell size analyses tissue sample was fixed overnight in 4% paraformaldehyde (PFA) and embedded in paraffin for histological sections.
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