C. elegans brood counts and male frequencies

MC Michelle A Carmell
GD Gregoriy A Dokshin
HS Helen Skaletsky
YH Yueh-Chiang Hu
JW Josien C van Wolfswinkel
KI Kyomi J Igarashi
DB Daniel W Bellott
MN Michael Nefedov
PR Peter W Reddien
GE George C Enders
VU Vladimir N Uversky
CM Craig C Mello
DP David C Page
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Brood and male frequency counts were performed at 20 and 25°C. Briefly, animals were single picked at mid-L4 stage and followed with daily transfers until they produced no more progeny. Animals were counted and males were scored when the population on a progeny plate reached adulthood.

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