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CAF cancer cells co‐cultures and conditioned media preparation
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Co‐culture is based on the combined culture of 1:1 mix of PANC‐1 and P‐CAFs in transwell format (BD Biosciences 0.4 μm pore size). Conditioned medium (CM) was prepared from CAFs or tumor cells grown in 35 mm culture plates in serum‐free media (1 ml) for 48 h. CM were collected, centrifuged (450 g, 4 min), and incubated with cancer cells for the indicated time points.
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This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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