NCI-H358 xenograft model

NCI-H358 cells (2 × 10⁶) resuspended in 100 μL PBS:Matrigel were injected subcutaneously into the flanks of nude mice. Tumour growth was assessed twice weekly by calliper measurement and mice were randomized to treatment arms when tumours reached 150 mm3 (using the formula: width2 x length x 0.5). Carboplatin (60 mg/kg) was delivered by a single tail-vein injection. Dactolisib was prepared in 10% DMSO:90% PEG300 and administered twice-weekly at 45 mg/kg by oral gavage. All in vivo experiments, procedures and endpoints were approved by the Garvan Institute of Medical Research Animal Ethics Committee.