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KG Katharina Gimpl
JK Jessica Klement
SK Sandro Keller
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To ensure good separation by SEC, the sample volume should be as small as possible and should not exceed the maximum volume provided by the manufacturer (typically, 0.5–2 % of the total column volume). We usually apply 50–100 μL of sample to a column of ~24 mL.

Copyright and License information: Gimpl et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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