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Healthy and SSc fibroblasts were plated at 2 × 104 cells/mL in 96-well tissue culture plates. Cell culture supernatants were assayed for IL-6 and IL-8 by magnetic bead-based multiplex assay according to the manufacturer’s protocol. Data acquisition was performed by a Bio-Plex 200 System™ (Bio-Rad Laboratories, Inc., Hercules, CA, USA), which uses Luminex fluorescent bead-based technology. Data analysis was performed by Bio-Plex Manager™ 6.0 software (Bio-Rad Laboratories, Hercules, CA, USA). Cell supernatants were run in triplicate.
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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