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GBM cells after LINC01614 siRNAs transfection were seeded into six-well plates (800 cells/well). Colonies were allowed to form for about two weeks in RPMI-1640 media (with 10% FBS) in an incubator with 37°C and 5% CO2. Then, the colonies were washed and treated with methanol (100%) and crystal violet (0.2%) for a quarter. After washing with PBS twice, the colonies were photographed by a microscope.
Copyright and License information: ©2020 Wang et al.
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